| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
22/03/2008 |
|
Data da última atualização: |
28/06/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ASSIS, C. R. D.; AMARAL, I. P. G.; CASTRO, P. F.; CARVALHO JÚNIOR, L. B.; BEZERRA, R. S. |
|
Título: |
Effect of Dichlorvos on the acetylcholinesterase from tambaqui (Colossoma macropomum) Brain. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Environmental Toxicology and Chemistry, v. 26, n. 7, p. 1451-1453, 2007. |
|
ISSN: |
0730-7268 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Dichlorvos is an acutely toxic organophosphorous pesticide that is known as a classical acetylcholinesterase (AChE; EC 3.1.1.7) inhibitor. Here, the brain AChE from the important Amazonian fish tambaqui (Colossoma macropomum) was assayed in the presence of this insecticide and also of deltamethrin, a classical sodium and potassium channel inhibitor (negative control). Four tissue homogenates were analyzed in triplicate for AChE activity using acetylthiocholine as the substrate and 5,5'-dithiobis(2-nitrobenzoic) acid (DTNB) as the color-developing agent. Each tissue homogenate represented pooled brains from five fish. The inhibitory effect of dichlorvos on AChE activities was determined at concentrations from 0.001 to 10 ppm and compared to controls. This effect followed an exponential decay model (y = 9.420 + 26.192e(-x/5.380); r2 = 0.989), presenting IC50 (the concentration of dichlorvos that is required for 50% of AChE inhibition) of 0.081 ppm (0.368 umol/L). No effect was observed for the deltamethrin, and the concentration 0.0452 umol/L of dichlorvos was significantly different from this control. These results suggest that tambaqui brain AChE can be proposed as a biomarker for dichlorvos and can be used as a tool for aquatic environment monitoring. |
|
Palavras-Chave: |
Biomarker; Organophosphorous pesticide. |
|
Thesagro: |
Colossoma Macropomum; Tambaqui. |
|
Thesaurus Nal: |
acetylcholinesterase; dichlorvos. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24534/1/5620260715-ftp.pdf
|
|
Marc: |
LEADER 02018naa a2200253 a 4500
001 1069727
005 2022-06-28
008 2007 bl uuuu u00u1 u #d
022 $a0730-7268
100 1 $aASSIS, C. R. D.
245 $aEffect of Dichlorvos on the acetylcholinesterase from tambaqui (Colossoma macropomum) Brain.
260 $c2007
520 $aDichlorvos is an acutely toxic organophosphorous pesticide that is known as a classical acetylcholinesterase (AChE; EC 3.1.1.7) inhibitor. Here, the brain AChE from the important Amazonian fish tambaqui (Colossoma macropomum) was assayed in the presence of this insecticide and also of deltamethrin, a classical sodium and potassium channel inhibitor (negative control). Four tissue homogenates were analyzed in triplicate for AChE activity using acetylthiocholine as the substrate and 5,5'-dithiobis(2-nitrobenzoic) acid (DTNB) as the color-developing agent. Each tissue homogenate represented pooled brains from five fish. The inhibitory effect of dichlorvos on AChE activities was determined at concentrations from 0.001 to 10 ppm and compared to controls. This effect followed an exponential decay model (y = 9.420 + 26.192e(-x/5.380); r2 = 0.989), presenting IC50 (the concentration of dichlorvos that is required for 50% of AChE inhibition) of 0.081 ppm (0.368 umol/L). No effect was observed for the deltamethrin, and the concentration 0.0452 umol/L of dichlorvos was significantly different from this control. These results suggest that tambaqui brain AChE can be proposed as a biomarker for dichlorvos and can be used as a tool for aquatic environment monitoring.
650 $aacetylcholinesterase
650 $adichlorvos
650 $aColossoma Macropomum
650 $aTambaqui
653 $aBiomarker
653 $aOrganophosphorous pesticide
700 1 $aAMARAL, I. P. G.
700 1 $aCASTRO, P. F.
700 1 $aCARVALHO JÚNIOR, L. B.
700 1 $aBEZERRA, R. S.
773 $tEnvironmental Toxicology and Chemistry$gv. 26, n. 7, p. 1451-1453, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | GILABEL, A. P.; SANTOS, P. M.; ANDRADE, A. S.; PEZZOPANE, C.; MONTEIRO, F. A. Caracterização de acessos Paspalum para sistemas intensivos de produção no Estado de São Paulo:exigências nutricionais. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. p. 29. (Embrapa Instrumentação. Documentos, 57) Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
